TABLE 1.
Oligonucleotide primers used in this work
| Primer | Orientation, 5′→3′ | Sequence | Use |
|---|---|---|---|
| ERG3C | Antisense | GCAGGCGTTTGTAGATGG | Specific for erg3A |
| ERG3D | Sense | ATGGACGTTGTGCTTGAC | Specific for erg3A |
| ERG3F | Sense | ATACCTACCTTCAGTACC | Specific for erg3A |
| ERG3Z | Antisense | CTCAGGAAGTTTTCTTGG | Specific for erg3A |
| ERG321 | Sense | GCCAAACAATCAAGGCACAG | Specific for erg3A |
| ERG322 | Antisense | GATCTATGGTCGGTTGGTTG | Specific for erg3A |
| ERG3B1 | Sense | CGGCACCATGGATATTG | Specific for erg3B |
| ERG3B2 | Antisense | AGATGTAGGAGAGGGTTG | Specific for erg3B |
| ERG3B3 | Sense | ATACCGTTGCCATTCTATTG | Specific for erg3B |
| ERG3B4 | Antisense | CGCAACGGAATACGATATTG | Specific for erg3B |
| ERG3B5 | Sense | CGAGGAAGTCGATCAAAG | Specific for erg3B |
| ERG3B9 | Sense | ATCCCCATGGAGATTGCG | Specific for erg3B |
| ERG3B10 | Antisense | GTCGTGAATCATTACCGTC | Specific for erg3B |
| ERG3B6 | Sense | GACAGAGTAGGCACGTAG | Specific for erg3B |
| ERG3B7 | Antisense | CCCCTTATCTGGCTGACA | Specific for erg3B |
| ERG3C1 | Sense | ATCCTCACGCTGCCATGG | Specific for erg3C |
| ERG3C2 | Antisense | GTCGCCGTCGTGAATGAG | Specific for erg3C |
| ERG3C3 | Sense | ATGGATGTCGCTCTCGAG | Specific for erg3C |
| ERG3C4 | Antisense | TTGAAGTCCCTCACTCCC | Specific for erg3C |
| ERG3C5 | Sense | AGGATATAAATACCTCCG | Specific for erg3C |
| ERG3C6 | Antisense | CTAATCGTCATGCATATG | Specific for erg3C |
| T-7 | Universal | TAATACGACTCACTATAGGGCGA | Clone sequencing |
| Sp-6 | Reverse | ATTTAGGTGACACTATAGAATAC | Clone sequencing |
| ERG3Akpn1 | Sense | CCTTCAGGTACCCGCTC | Specific for erg3A |
| ERG3Akpn2 | Antisense | GAGCGGGTACCTGAAGG | Specific for erg3A |
| ERG3BKpn1 | Sense | CAAGTTGGTACCGACTCC | Specific for erg3B |
| ERG3BKpn2 | Antisense | GGAGTCGGTACCAACTTG | Specific for erg3B |
| HygKpn1 | Sense | GGTACCTGATATTGAAGG | Specific for hyg |
| HygKpn2 | Antisense | GGTACCTTAACTGGTTCC | Specific for hyg |
| Tub1 | Sense | AACCAAATTGGTGCCGC | Specific for tub1 |
| Tub2 | Antisense | CACGGATCTTGGAGATC | Specific for tub1 |