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(A) SDS-PAGE of fluorescently labeled bacteriorhodopsin S35C-Alexa488. (Lanes 1 and 4) Molecular weight marker, (lane 2) undigested bacteriorhodopsin S35C-Alexa488, (lane 3) partially digested bacteriorhodopsin S35C-Alexa488. The AB fragment in lane 3 is marked by a white arrow. (B) Secondary structural model of the AB-helix fragment of bacteriorhodopsin. The amino acids are shown in single letter code.
