FIGURE 4.

(A) Calculated fluorescence anisotropy decays (solid lines) for Alexa488 in water (blue) and methanol (green) are compared to the measured anisotropy decays (dashed lines). (B and C) Time courses of the fluorescence intensities I_‖(t) (red) and I_⊥(t) (blue) in water (B) and methanol (C). IRF is the instrument response function. The residuals of the fit for I_‖(t) and I_⊥(t) are shown as insets. The excitation was at 470 nm. The fluorescence emission was detected after passing through a cutoff color glass filter OG 515.