TABLE 1.

Capacity of HCV-specific HLA-A2.1-restricted peptide epitope to bind to purified recombinant HLA-A2.1 molecules and to induce specific CTL or IFN-γ-producing T cells after direct injection

Gene	Residue (aa)	Epitope		Binding (% inhibition)a	ELISPOT (no. of specific spots/106 cells)b				Specific lysis (%) (E/T = 33)c
		Sequence	Name		Mc	M1	M2	M3	Mc	M1	M2	M3
C	35-44	YLLPRRGPRL	C35-44	58	12	73	160	73	2	5	23	2
	132-140	DLMGYIPLV	C132-140	71	0	211	206	284	4	44	19	26
E1	220-227	IMHTPGCV	E1 220-227	55	0	0	55	0	1	2	0	14
	257-266	TIRRHVDLLV	E1 257-266	0	ND	ND	ND	ND	ND	ND	ND	ND
	363-372	SMVGNWAKV	E1 363-372	16	0	172	160	328	0	47	3	66
E2	401-411	SLVSWLSQGPS	E2 401-411	30	0	0	0		0	0	0
	614-622	RLWHYPCTI	E2 614-622	92	0	82	47	200	10	34	26	22
	686-694	ALSTGLIHL	E2 686-694	100	0	403	260	112	2	65	10	79
	725-734	FLLLADARV	E2 725-734	66	0	333	215	622	0	83	34	36
NS3	1073-1081	CVNGVCWTV	NS3 1073-1081	18	0	5	47	13	2	27	88	0
	1169-1177	LLCPSGHVV	NS3 1169-1177	77	2	137	322	160	9	45	7	53
	1406-1415	KLTGLGLNAV	NS3 1406-1415	71	0	188	458	230	18	33	15	40
	1585-1593	YLVAYQATV	NS3 1585-1593	76	0	0	173	190	11	7	19	16
NS4B	1769-1777	HMWNFITGI	NS4B 1769-1777	30	0	162	93	30	0	13	35	0
	1789-1797	SLMAFTASI	NS4B 1789-1797	63	71	61	157	177	20	31	15	38
	1807-1816	LLFNILGGWV	NS4B 1807-1816	94	0	605	22	658	0	19	12	0
	1851-1859	ILAGYGAGV	NS4B 1851-1859	32	0	5	2	0	6	0	0	0
NS5A	2221-2231	SPDADLIEANL	NS5A 2221-2231	37	42	102	0	8	4	0	2	2
	2252-2260	ILDSFDPLR	NS5A 2252-2260	34	0	37	0	30	8	0	3	8
NS5B	2578-2587	RLIVFPDLGV	NS5B 2578-2587	100	49	234	116	305	12	41	33	46
	2594-2602	ALYDVVSTL	NS5B 2594-2602	80	39	261	331	326	0	81	44	2
	2727-2735	KLQDCTMLV	NS5B 2727-2735	99	25	255	277	297	13	38	52	60

^aPercent inhibition of binding of the FLP-control peptide onto purified HLA.A2.1 molecules for a ratio competitor peptide/control peptide = 30.

^bND, not done. Mc, control mouse; M1, M2, and M3, immunized mice.

^cPercent specific lysis at an E:T ratio of 1:33.