TABLE 2.

Capacity of HCV-DNA vaccines encoding individual antigens to induce specific CTL and IFN-γ-producing T cells

Plasmid	Epitope	ELISPOT (no. of specific spots/106 cells)				Specific lysis (%) (E/T = 33)b
		Mca	M1	M2	M3	Mc	M1	M2	M3
pgWizCore	C35-44	0	0	0	0	10	20	9	0
	C132-140	154	650	390	400	5	44	39	70
pgWizE1	E1 220-227	3	66	29	31	4	1	10	5
	E1 257-266	0	76	38	12	23	22	3	5
pgWizE2	E2 401-411	5	25	60	90	12	42	39	37
	E2 614-622	15	65	180	120	10	29	54	55
pgWizNS3	NS3 1073-1081	10	120	475	120	16	43	48	34
	NS3 1169-1177	10	120	90	145	13	31	22	13
	NS3 1406-1415	68	112	102	66	12	17	11	6
	NS3 1585-1593	56	90	66	69	9	23	12	23
pgWizNS4	NS4B 1769-1777	120	550	330	480	2	1	7	5
	NS4B 1789-1797	3	35	54	72	9	1	0	0
	NS4B 1807-1816	9	240	360	750	1	40	28	33
	NS4B 1851-1859	ND	ND	ND	ND	ND	ND	ND	ND
pgWizNS5A	NS5A 2221-2231	105	0	0	0	12	21	19	23
	NS5A 2252-2260	120	0	0	0	16	22	15	9
pgWizNS5B	NS5B 2578-2587	0	240	300	195	8	42	23	36
	NS5B 2594-2602	0	300	380	330	9	41	33	49
	NS5B 2727-2735	15	390	309	198	5	10	17	26

^aMc, control mouse; M1, M2, and M3, immunized mice. ND, not done.

^bPercent specific lysis at an E:T ratio of 1:33.