TABLE 3.
Comparative classification of HCV HLA-A2.1 epitopes according to their immunogenicity in different vaccination contexts
| Gene | Epitope | Vaccination strategyc
|
|||
|---|---|---|---|---|---|
| Peptide | DNAa | Adenovirusb | DNA-priming/adenovirus-boosting | ||
| C | C35-44 | Type 3 | Type 2 | Type 2 | Type 1 |
| C132-140 | Type 1 | Type 1 | Type 1 | Type 1 | |
| E1 | E1 220-227 | Type 3 | Type 3 | Type 3 | Type 3 |
| E1 257-266 | ND | Type 3 | Type 3 | Type 3 | |
| E1 363-372 | Type 1 | Type 2 | Type 2 | Type 2 | |
| E2 | E2 401-411 | Type 3 | Type 2 | Type 2 | Type 2 |
| E2 614-622 | Type 1 | Type 1 | Type 1 | Type 1 | |
| E2 686-694 | Type 1 | Type 1 | Type 1 | Type 1 | |
| E2 725-734 | Type 1 | Type 1 | Type 1 | Type 1 | |
| NS3 | NS3 1073-1081 | Type 2 | Type 1 | Type 1 | ND |
| NS3 1169-1177 | Type 1 | Type 1 | Type 4 | ND | |
| NS3 1406-1415 | Type 1 | Type 1 | Type 4 | ND | |
| NS3 1585-1593 | Type 4 | Type 3 | Type 3 | ND | |
| NS4B | NS4B 1769-1777 | Type 4 | Type 4 | ND | ND |
| NS4B 1789-1797 | Type 1 | Type 3 | ND | ND | |
| NS4B 1807-1816 | Type 4 | Type 4 | ND | ND | |
| NS4B 1851-1859 | Type 3 | ND | ND | ND | |
| NS5A | NS5A 2221-2231 | Type 3 | Type 3 | ND | ND |
| NS5A 2252-2260 | Type 3 | Type 3 | ND | ND | |
| NS5B | NS5B 2578-2587 | Type 1 | Type 1 | ND | ND |
| NS5B 2594-2602 | Type 1 | Type 1 | ND | ND | |
| NS5B 2727-2735 | Type 1 | Type 1 | ND | ND | |
Combined data from immunizations performed with plasmids expressing single (Table 2) or multiple (Core-E1-E2 or NS3-NS4; Fig. 2) genes. Optimal immunogenicity that could be observed for each epitope with either a single or combined gene vaccination is indicated.
Data obtained after one immunization with recombination adenoviruses expressing Core, Core-E1-E2, or NS3 (data not shown; Fig. 3).
Type 1, able to induce both strong specific CTL (typically >30% lysis at on E:T ratio of 1:33) and high frequencies of IFN-γ-secreting cells (typically >100 for 106 cells, up to 650); type 2, able to induce very weak specific CTL (typically <30% at an E:T ratio of 1:33) and low frequencies of IFN-γ-secreting cells (typically <100 for 106 cells, down to 0); type 3, unable to induce specific CTL or IFN-γ-secreting cells; type 4, able to predominantly induce IFN-γ-secreting cells.