TABLE 4.
Amino acid sequence comparisons of HCV HLA-A2 epitopes sequences with counterparts derived from genotype 1b, 1a, and 4 isolates
| Protein | Epitope | % Conservation genotypea
|
||
|---|---|---|---|---|
| 1b | 1a | 4 | ||
| C | C35-44 | 95 (195/206) | 100 (56/56) | 100 (11/11) |
| C132-140 | 96 (197/206) | 97 (58/60) | 78 (8/11) | |
| E1 | E1 220-227 | 78 (178/229) | 0 (0/397) | 0 (0/15) |
| E1 257-266 | 83 (191/229) | 0 (0/397) | 0 (0/15) | |
| E1 363-372 | 84 (189/224) | 91 (365/401) | 0 (0/10) | |
| E2 | E2 401-411 | 0 (0/143) | 0 (0/391) | 0 (0/11) |
| E2 614-622 | 80 (115/143) | 88 (15/17) | 0 (0/1) | |
| E2 686-694 | 98 (138/141) | 94 (15/16) | 100 (1/1) | |
| E2 725-734 | 90 (127/141) | 94 (15/16) | 100 (1/1) | |
| NS3 | NS3 1073-1081 | 62 (84/136) | 0 (0/14) | 0 (0/1) |
| NS3 1169-1177 | 28 (38/136) | 0 (0/14) | 0 (0/1) | |
| NS3 1406-1415 | 15 (20/135) | 0 (0/14) | 0 (0/1) | |
| NS3 1585-1593 | 96 (130/135) | 100 (14/14) | 100 (1/1) | |
| NS4 | NS4B 1769-1777 | 0 (0/30) | 0 (0/14) | 0 (0/2) |
| NS4B 1789-1797 | 93 (28/30) | 100 (14/14) | 0 (0/2) | |
| NS4B 1807-1816 | 97 (29/30) | 100 (14/14) | 100 (1/1) | |
| NS4B 1851-1859 | 93 (28/30) | 100 (14/14) | 0 (0/1) | |
| NS5A | NS5A 2221-2231 | 83 (115/139) | 0 (0/14) | 0 (0/1) |
| NS5A 2252-2260 | 40 (56/139) | 0 (0/14) | 0 (0/1) | |
| NS5B | NS5B 2578-2587 | 96 (136/142) | 100 (14/14) | 0 (0/1) |
| NS5B 2594-2602 | 99 (141/142) | 0 (0/14) | 0 (0/1) | |
| NS5B 2727-2735 | 87 (123/142) | 0 (0/14) | 0 (0/1) | |
a
That is the number of isolates presenting a homology of 100% with the epitope sequences used in our study (derived from the HCV-JA isolate)/the total number of isolate sequences aligned.