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. 2005 Sep 23;89(6):3960–3975. doi: 10.1529/biophysj.105.060731

FIGURE 5.

FIGURE 5

Lqh-venom inhibits CFTR intraburst activity. (A) Representative trace of WT-CFTR from an excised inside-out patch with 1 mM MgATP and 50 U/mL PKA continuously present; Vm = −100 mV. Note that brief closings of at least 5 ms in duration occur frequently during an open burst. (B) Representative trace of WT-CFTR with 1 mM MgATP, 50 U/mL PKA, and 0.1 mg/mL Lqh-pf venom continuously present; Vm = −100 mV. Fewer brief closings are evident (lower trace); however, overall burst appearance is altered due to the introduction of venom-induced closings (evident in middle trace). CFTR channel activity was filtered at 500 Hz and acquired by computer at 2 kHz. Dashed line represents the closed current level, and downward deflections represent channel openings.