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. 2005 Sep 23;89(6):3960–3975. doi: 10.1529/biophysj.105.060731

FIGURE 7.

FIGURE 7

Venom-mediated intraburst inhibition of single CFTR channels under different experimental conditions, in excised inside-out patches. (A) Representative trace of WT-CFTR with 1 mM MgATP, 50 U/mL PKA, and 5 mM AMP-PNP continuously present before and during application of 0.2 mg/mL Lqh-pf venom. (B) Representative trace of WT-CFTR with 1 mM MgATP, 50 U/mL PKA, and 2.75 mM vanadate continuously present before and during application of 0.2 mg/mL Lqh-pf venom. (C) Representative trace of K1250A-CFTR with 1 mM MgATP and 50 U/mL PKA continuously present before and during application of 0.1 mg/mL Lqh-pf venom. Before binding of AMP-PNP (A top, left) or vanadate (B top, left) closings from normal channel gating are easily discernable. All records at Vm = −100 mV.