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. 2005 Sep 30;89(6):4129–4138. doi: 10.1529/biophysj.105.062695

FIGURE 6.

FIGURE 6

Competitive binding assay of D210N-APE1 to DNA containing either (A) coumarin:THF or (B) guanine:THF. Lane 1 shows the labeled DNA in the absence of the protein. Lane 2 shows the protein binding in the absence of competitor (18% A, 98% B). Lane 3 shows binding in the presence of 100-fold excess of unlabeled, specific oligodeoxynucleotide duplex that has THF opposite coumarin (5%, A) or guanine (3%, B). Lane 4 shows the binding in the presence of 100-fold excess of unlabeled, nonspecific competitor DNA that has guanine opposite cytosine (22%, A) (97%, B).