FIGURE 3.

Trp fluorescence intensity (open circle) of GCN4-p1c″ at 350 nm as a function of temperature. The value λ_ex = 270 nm. The solid line is a two-state fit using the method of Privalov and co-workers (21). The resulting thermodynamic parameters, i.e., ΔH_m = 19.3 kcal mol⁻¹, ΔS_m = 60 cal K⁻¹ mol⁻¹, ΔC_p = 40 cal K⁻¹ mol⁻¹, and T_m = 49.9°C, are similar to those obtained by Dragan and Privalov on GCN4-p1 (21).