TABLE 1.

Experimental comparison of TD- and FD-FLIM data analysis

	Lifetime (ns)		CV (%)		CV increase
	TD	FD	TD	FD	TD	FD
Control	1.90 ± 0.06	1.95 ± 0.03	23 ± 8	36 ± 4	–	–
Bleached	1.40 ± 0.06	1.40 ± 0.03	39 ± 8	58 ± 3	1.7	1.6
Nonbleached	1.74 ± 0.06	1.64 ± 0.04	27 ± 10	50 ± 3	1.2	1.4

	ɛ (%)		α (%)
	TD	FD	TD	FD
Bleached	56 ± 4	69 ± 3	52 ± 5	45 ± 3
Nonbleached	61 ± 10	71 ± 4	22 ± 4	28 ± 3

Average values refer to the fluorescent bead experiments of Fig. 5. The errors are presented as standard deviations.