FIGURE 2.

Effect of sucrose on single-channel currents of wild-type (Cx46 and Cx32E₁43) and chimeric channels (Cx32M₁46E₁43 and Cx46M₁32). Representative recordings of the same channel contained in an inside-out membrane patch before (top trace) and with (bottom trace) 100 mM sucrose perfusion are shown. Open (o) and closed (c) states are indicated by dotted lines. All-point amplitude histograms and single-channel conductance are shown on the side of each record. To facilitate recognition of the subconductance state (s), part of the recordings in Fig. 2 A (bars) are shown in Fig. 2 B at extended scale.