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. 2005 Oct 7;90(1):164–172. doi: 10.1529/biophysj.105.063503

FIGURE 1.

FIGURE 1

Surface representations of 3D cryo-EM reconstructions of RyR2 with and without bound FKBP12.6. (A) The 3D map of RyR2 (+FKBP), obtained by assembly in vitro of purified RyR2 incubated with FKBP12.6 alone, and (B) the 3D map of RyR2 (−FKBP), obtained by incubating RyR2 with FKBP12.6 and excess FK506, with the superimposed map of difference density (shown in blue), as obtained by subtracting the 3D map of RyR2 (−FKBP) from that of RyR2 (+FKBP). In panel A, arrows point to the extra density in RyR2 (+FKBP12.6) that accounts for the FKBP12.6 mass that is enhanced in the difference map shown in panel B. The 3D volumes for the two conditions are each shown in three views: left, cytoplasmic view; middle, SR junctional face; and right, side view. Abbreviation: TA, transmembrane assembly. Numerals refer to established nomenclature of domains. Scale bar ∼100 Å.