TABLE 4.

Abundance of HSV-1 transcripts at 4 h after infection with RP5 virus in HeLa cells treated with HMBA^a

Transcript set	Kinetic class and IDb	Abundance			Wt %d	t test valuee
		Medianc	SD	%
RICP0	IE-1	13,130	5,830	3.2	4.1	0.04
U54	IE-2	42,970	11,840	10.5	4.1	0.03
RICP4	IE-3	21,430	11,580	5.2	4.0	0.32
R/S22	IE-4	27,730	6,770	6.8	4.8	0.16
U4-5′	E-1	810	930	0.2	0.1	0.20
U4/5	E-2	4,680	2,140	1.1	2.0	0.27
U8/9	E-3	880	1,090	0.2	1.3	0.08
U8-5′	E-4	2,410	1,360	0.6	0.4	0.44
U21	E-5	880	530	0.2	0.7	0.17
U23	E-6	13,670	1,810	3.3	3.1	0.47
U29	E-7	10,630	6,730	2.6	2.0	0.12
U30	E-8	10,770	6,930	2.6	2.9	0.99
U37	E-9	520	630	0.1	0.8	0.18
U39-5′	E-10	13,480	4,240	3.3	1.9	0.15
U39/40	E-11	13,220	4,030	3.2	4.8	0.11
U42	E-12	6,920	2,750	1.7	3.1	0.21
U43	E-13	2,300	690	0.6	0.8	0.13
U50	E-14	10,020	6,220	2.4	3.0	0.99
U52-5′	E-15	2,230	1,120	0.5	0.2	0.11
U55	E-16	1,520	740	0.4	0.7	0.18
U56	E-17	3,350	630	0.8	1.6	0.02
US2	E-18	1,360	330	0.3	0.7	0.25
U1	L-1	10,200	2,210	2.5	2.6	0.83
U3	L-2	850	520	0.2	0.6	0.21
U10	L-3	3,060	1,130	0.7	0.8	0.63
U16/17	L-4	1,980	400	0.5	0.8	0.30
U15	L-5	670	1,340	0.2	0.3	0.83
U18/20	L-6	4,510	2,300	1.1	1.5	0.31
U19/20	L-7	2,470	890	0.6	1.5	0.15
U19-5′	L-8	2,600	1,190	0.6	0.4	0.24
U22	L-9	1,500	510	0.4	0.6	0.29
U24	L-10	2,600	1,280	0.6	0.6	0.77
U25	L-11	3,350	1,360	0.8	1.0	0.30
U27/8	L-12	8,610	3,280	2.1	2.7	0.43
U27-5′	L-13	9,460	2,840	2.3	1.5	0.18
U31/34	L-14	2,850	740	0.7	0.5	0.12
U35	L-15	3,840	840	0.9	1.7	0.27
U38	L-16	1,190	750	0.3	0.5	0.30
U41	L-17	510	170	0.1	0.3	0.15
U44-5′	L-18	1,610	640	0.4	0.3	0.19
U44/45	L-19	5,710	1,330	1.4	2.2	0.18
U46/47	L-20	11,800	3,770	2.9	2.0	0.04
U48	L-21	730	750	0.2	1.6	0.00
U51	L-22	5,760	1,760	1.4	1.2	0.65
RLXY	L-23	480	350	0.1	0.2	0.22
RLX	L-24	6,540	2,130	1.6	0.7	0.01
RICP34.5	L-25	2,850	1,520	0.7	0.7	0.92
US5-5′	L-26	2,010	1,290	0.5	0.6	0.79
US8-5′	L-27	3,590	1,120	0.9	0.8	0.72
US8/9	L-28	26,120	10,630	6.4	4.2	0.07
RLAT-5′	LT-1	580	670	0.1	0.2	0.66
RHA6	LT-2	250	320	0.1	0.2	0.26
RLATX	LT-3	1,590	1,510	0.4	0.2	0.12
RLAT-3′	LT-4	3,750	2,800	0.9	0.4	0.04
U1X	?	130	120	0.0	0.1	0.20
U6/7	L/?	2,450	770	0.6	1.5	0.10
U11/13	L/E/L	4,150	2,820	1.0	2.1	0.22
U36	E/L	350	220	0.1	0.4	0.20
U43.5-5′	?	260	150	0.1	0.0	0.14
U49	E/L	14,880	7,440	3.6	2.7	0.04
U52/53	E/L	3,170	2,240	0.8	1.3	0.01
RLAT-1	LAT	890	560	0.2	0.1	0.14
ROP	?	1,740	830	0.4	0.1	0.01
US3-5′	L?	8,360	3,340	2.0	1.0	0.22
US3/4	E/L?	3,130	2,180	0.8	2.4	0.10
US5/6/7	EL/E/E	16,570	7,360	4.0	3.6	0.27
US10/11/12	E/E/IE	15,730	6,790	3.8	4.4	0.96
Total		410,340

^aInfection was initiated at an MOI of 0.05 PFU (50 virions) per cell in the presence of 5 mM HMBA. Bold entries represent either single transcripts or multiple transcripts of the same kinetic class.

^bSee Table 1, footnote b.

^cMedian values are based upon eight separate experiments. All values were determined at a laser power of 80 with a photomultiplier at 75%.

^dTaken from Table 1.

^eStudent's two-tailed t test (assuming unequal variance) was used to compare the relative abundance values of transcripts in all experiments of RP5-HMBA infection with corresponding set of values for wt infection at 4 hpi (Table 1). The null hypothesis is that the values are identical.