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. 2005 Oct 7;90(1):228–237. doi: 10.1529/biophysj.105.067066

FIGURE 2.

FIGURE 2

AFM images and section analyses (the dotted lines denote the location of the sections) of phase-separated supported lipid bilayers. Lighter shading represent higher surfaces. (A) Supported lipid bilayer made by Method A. The measured domain heights extend ∼1.8 nm above the surrounding DLPC fluid-phase matrix. (B) Supported lipid bilayer made by Method B′. The bilayers prepared in this manner contained domains with areas extending 1.8 nm and 1.1 nm above the surrounding DLPC matrix. (Inset) 1.8 nm domains convert into 1.1 nm domains after ∼4 h; time after supported lipid bilayer formation: left, 30 min, middle, 1.5 h, and right, 4 h. (C) Supported lipid bilayer made by Method B. The domains are ∼1.1 nm higher than the fluid-phase region.