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. 2005 Oct 7;90(1):238–249. doi: 10.1529/biophysj.105.073403

FIGURE 4.

FIGURE 4

Interfacial adsorption of SP-B-derived peptides. (a–c) Interfacial adsorption kinetics of SP-B synthetic peptides to a clean air-liquid interface from a buffered subphase (5 mM Tris pH 7, containing 150 mM NaCl). After 5-min equilibration, 10 μL of methanol containing the indicated amounts of peptides (in μg) were injected into the subphase and changes in surface pressure were monitored. The experiments were run at 25°C with continuous stirring of the subphase. Control experiments, performed by injecting the same volume of methanol in the absence of peptide, indicated no significant changes in surface pressure (not shown). (d,e) Increment of surface pressure 1 min (d) or 20 min (e) after injection of the different peptides into the subphase, plotted versus peptide concentration.