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Critical pressure for insertion of SP-B-derived peptides in phospholipid films. Increment of surface pressure (Δπ) observed in preformed DPPC/POPG (7:3, w/w) monolayers measured 20 min after injection of SP-B synthetic peptides (4 μg) into the subphase, plotted versus the initial surface pressure of the films (πi). The critical insertion pressure (πc) was calculated by extrapolation of the plot at Δπ = 0 mN/m.
