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. 2002 Dec;76(24):13077–13082. doi: 10.1128/JVI.76.24.13077-13082.2002

FIG. 4.

FIG. 4.

Effect of IL-7 on cell cycle status of quiescent human lymphocytes. Quiescent CD45RA−/CD4−, CD45RA−/CD8−, CD45R0−/CD4−, and CD45R0−/CD8− lymphocytes were isolated, and cultured under the following stimulation conditions: unstimulated, IL-7, IL-2, or anti-CD3 and anti-CD28 costimulation. After 3 days, cells were stained to detect DNA (y axis) and RNA (x axis) and analyzed to determine cell cycle status. Control stimulations performed in the presence of cell cycle inhibitor (N-acetyl butyric acid) and were used to set quadrant positions (bottom row). Seven similar experiments were performed.