FIGURE 14.

Confidence intervals for ζ₂ from the analysis of the spectra of the three spin-labeled double cysteine mutants of T4L using the tether-in-a-cone model. The values of χ² were obtained by adjusting ψ₁, ψ₂, and μ_max to obtain the best fit to the data at fixed values of ζ₂. The top panel shows the results plotted fullscale. The bottom panel shows an expanded scale. In both panels, each curve is scaled to its 99% confidence level calculated using the F-statistic (dashed line). Results are shown for T4L 61/65 (▪), T4L 65/68 (•), and T4L 65/69 (▴).