FIGURE 4.

Single channel recordings of RyR1-D⁴⁹³⁸N. (A) Single channel currents were recorded at indicated holding potentials in symmetrical 250 mM KCl with 2–20 μM Ca²⁺ and 1 mM ATP in the cis chamber as downward (left) or upward (right) inflections from the closed state (c–). (B–D) After addition of 500 nM ryanodine (B) and in subsequent presence of 500 nM cis (C) or 2 μM trans neomycin (D).