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. 2005 Nov 4;90(2):470–479. doi: 10.1529/biophysj.105.068213

FIGURE 4.

FIGURE 4

Time course of lipid mixing of liposomes (LUV) induced by peptide 97,848. The peptide is added at different concentrations (from 30 μM to 600 μM corresponding to peptide to lipid molar ratio from 1:50 to 2:5) to a mixture (1:4 w/w ratio) of R18-labeled and unlabeled LUVs. Increase of the R18 relative fluorescence due to probe dilution is followed at room temperature. The SIV tilted peptide at 150 μM is used as positive control, the addition of TFE (1.6% final concentration) as negative control. TFE (blank), •; and SIV peptide at 150 μM (positive control), ⋄. Addition of peptide 97,848 at 30 μM (P/L molar ratio, 1:50), ○; 60 μM (P/L ratio 1:25), ▴; 150 μM (P/L ratio 1:10), ×; and 300 μM (P/L ratio 1:5), Δ.