TABLE 3.
Comparison of the experimental Rg with the calculated sDNA fitted parameters as a function of temperature using the cylindrical structural model
| Diameter (Å) | Length (Å) | Rg (Å) | X2, R2 | 3D model from LORES | |
|---|---|---|---|---|---|
| 25°C | 6.1 ± 0.1* | 29.8 ± 0.5 | 8.8 ± 0.1 (9 ± 1) | 0.09319, 0.6965 | |
| 34°C | 14.9 ± 0.4 | 28.3 ± 0.9 | 9.6 ± 0.1 (10 ± 1) | 0.1482, 0.4752 | |
| 43°C | 23.0 ± 0.5 | 21.9 ± 0.8 | 10.3 ± 0.1 (11 ± 1) | 0.2148, 0.4749 | |
| 53°C | 14.7 ± 0.5 | 30.5 ± 1.0 | 10.1 ± 0.1 (10 ± 1) | 0.2251, 0.2391 | |
| 71°C | 25.4 ± 0.7 | 68.2 ± 1.5 | 21.7 ± 0.4 (19 ± 2) | 0.1381, 0.6727 | |
| 80°C | 22.5 ± 0.6 | 78.3 ± 1.0 | 24.3 ± 0.3 (24 ± 2) | 0.1686, 0.5884 |
The deviation is calculated from running LORES 15 times with same criteria. The values in parentheses are the experimental Rg values.