FIGURE 2.

Fluorescence properties of SOD derivatives. (A) Fluorescence emission spectra recorded for SOD derivatives, 100 μg/mL in 20 mM MOPS, pH 7 at 25°C, 280 nm excitation: 1), Mn²⁺ complex; 2), apo-protein; 3), Co²⁺ complex; and 4), Mn³⁺ complex. (B) Temperature dependence of fluorescence intensity for SOD derivatives, 100 μg/mL in 20 mM MOPS pH 7, 280 nm excitation as described in Experimental Procedures: 1), Mn²⁺ complex; 2), apo-protein; and 3), Co²⁺ complex.