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. 2002 Dec;76(24):12423–12434. doi: 10.1128/JVI.76.24.12423-12434.2002

FIG. 5.

FIG. 5.

(A) Capacity for expansion after 10 days of peptide stimulation of HBV and HCV tetramer-positive cells. PBMC from patients with acute HBV and HCV infection were stimulated with HBV core 18-27, envelope (ENV) 335-343, and polymerase (POL) 575-583 and with HCV NS3 1073-1081, NS3 1406-1415, and NS4B 1992-2000 peptides, respectively. Each bar represents the expansion after 10 days of stimulation. The capacity for expansion in the two groups were significantly different by Student's t test. (B) Analysis of the perforin contents of HBV- and HCV-specific CD8 cells from short-term polyclonal CTL lines. Each bar represents the percentage of perforin-positive cells after 10 days of peptide stimulation among the overall population of HBV- or HCV-specific CD8 lymphocytes. (C and D) Functional characterization of HBV- and HCV-specific short-term CTL lines assessed as IFN-γ production (C) and cytolytic activity (D). Virus-specific CD8 cells were analyzed after 10 days of peptide stimulation in patients with acute HBV and HCV infection. ∗, frequency of tetramer-positive cells was <0.01% of CD8+ cells; nt, not tested.