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. 2005 Nov 18;90(2):L17–L19. doi: 10.1529/biophysj.105.075176

TABLE 1.

Comparison of apparent analytically and numerically calculated L and D, and corrected ones, to their real values for different temporal regimes

τ/T Lm/L Lm,s/L Lc/L Dm/D Dm,s/D Dc/D
10 0.984 0.984 1.000 0.936 0.933 0.996
6.4 0.975 0.958* ND 0.903 ND ND
2 0.923 0.924 1.000 0.730 0.721 0.983
1 0.858 0.859 0.998 0.552 0.542 0.967
0.5 0.753 0.755 1.002 0.341 0.342 1.002
0.333 0.675 0.676 1.026 0.228 0.235 1.136
0.0048 0.10 0.13* ND 0.010 ND ND

τ is the equilibriation time and T the detector exposure time. Parameters without index are real ones. The index “m” denotes an analytically calculated apparent parameter; “m,s” a numerically calculated one. The index “c” denotes a corrected value (ideally equal to the real one) obtained from the numerically calculated one using Eqs. 12, 13, and 6. ND, not determined (because D2-4 measured in Ritchie et al. (1) cannot be used in this framework).

*

Data from Figs. 2 and 3 C of Ritchie et al. (1).