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. 2005 Nov 11;90(3):1098–1106. doi: 10.1529/biophysj.105.070672

FIGURE 9.

FIGURE 9

(a) Gel array containing three horizontal lanes with fluorescent bands indicating 58 bp dsDNA found at pH 3 in the compartments housing the negative (−) and positive (+) electrodes in a bi-cell with a 1.8 × 2.2 nm2 pore in a 20 nm thick membrane as shown in Fig. 1 d (along with a 100 bp ladder.) The 58 bp permeates the pore only for V > 4.00 V for pH 3. (b) Similar to a but instead using pH 4. The 58 bp permeates this pore only for V > 5.0 V for pH 4. (c) Similar to a but instead using pH 8.5. The 58 bp permeates this pore only for V > 5.75 V for pH 8.5. (d) qPCR results obtained for the same pore showing the copy number versus voltage. A total of 622 bp dsDNA permeates the pore for V > 3.75 V for pH 3, V > 4.75 V for pH 4, and V > 5.0 V for pH 8.5.