FIG. 3.

Pulse experiments with endosome- and proteasome-interfering drugs. A9 cells were infected with MVMp at an MOI of 10 for 1 h at 4°C to allow viral attachment but not internalization, followed by washing to remove unbound virus, and were further incubated at 37°C. At 1-h intervals from 0 to 10 h postinfection, BFA (5 μg/ml), BA (150 nM), or MG132 (25 μM) was added to the medium. Control cells were not treated with any drug. The cells were incubated until 18 h postinfection and trypsinized, and total DNA was extracted and quantified as described in Materials and Methods. The MVM DNA content in nontreated cells is represented by a dotted line. Values represent the mean of three independent experiments.