Skip to main content
. 2006 Feb;80(4):1886–1896. doi: 10.1128/JVI.80.4.1886-1896.2006

FIG. 7.

FIG. 7.

HIV-1 replication is impaired in LEDGF/p75 knockdown cell lines. (A) HeLaP4, HeLaP4/shEGFP, HeLaP4/shp75PC, HeLaP4/shp75PC/BCCl3, HeLaP4/shp75mutCl1, HeLaP4/shp75Cl15, and HeLaP4/shp75Cl15/BCCla cells were infected with HIV-1(NL4.3) at an MOI of 0.01. p24 antigen production in the supernatants 60 h after infection was measured by ELISA. Average levels ± SD for four experiments are shown. (B) HeLaP4, HeLaP4/shp75Cl15, and HeLaP4/shp75Cl15/BCCla cells were infected with HIV-1(NL4.3) at an MOI of 0.002. The cells were subsequently trypsinized at day 3 postinfection and reseeded into a six-well plate. Supernatants were harvested on days 3, 6, and 9 postinfection, and p24 antigen production was measured. The inset highlights the p24 values after 3 days.