FIG. 2.

Analysis of 8-2/6/7-VLP protein content. 8-2/6/7-VLP of bovine RV origin were produced in the baculovirus expression system and purified from infected insect cell cultures by density gradient centrifugation in CsCl. Protein content of the purified VLP was analyzed by polyacrylamide gel electrophoresis with Coomassie blue staining (A) and Western blotting (B) with antiserum 8143 directed against bovine RV. 2/6/7-VLP and 8-2/6-VLP were used as controls. The positions of molecular size markers and locations of the individual RV proteins are indicated on the left and right, respectively. VP2* indicates a VP2 degradation product usually observed in VLP containing the VP2 and VP8-2 proteins.