FIG. 4.

GMTs of RV-specific antibodies after rectal immunization with 8-2/6/7-VLP. RV antibodies were tested by ELISA 15 days after the second rectal immunization. IgA and IgG RV antibody titers were determined for individual mice and used to calculate the GMT for each group of mice. For IgA-negative (<10) and IgG-negative (<100) samples, titers of 5 and 50, respectively, were used to calculate the GMT. (A) IgA GMT in saliva (black), stool (gray), and serum (white) samples. (B) IgG GMT in serum samples. For each group, formulations of vaccine are indicated along the x axis. Mice mock immunized with RPMI medium were used as a control. Error bars indicate standard errors of the geometric means. Asterisks indicate significant differences in pairwise comparisons of RV antibody titers between vaccinated and mock-immunized groups (Mann-Whitney U test, P < 0.05).