FIG. 1.

Full-length IE62 is differentially phosphorylated in the presence of ORF66 kinase. IE62 was expressed in the absence (A) or presence of ORF47 (B) or ORF66 kinase (C) in transfected 293T cells, metabolically labeled with [³²P]orthophosphate, immunoprecipitated, and subjected to tryptic phosphopeptide mapping as described in Materials and Methods. The X in the lower left corner of each map indicates the origin of spotting of the peptides. The horizontal axes represent electrophoresis of the peptides on TLC plates, and the vertical axes represent the chromatography step. Phosphopeptide spots are labeled with numbers to facilitate reference from panel to panel, and arrows in panel C indicate the differences in spots generated by coexpression of IE62 with the ORF66 kinase that are discussed in the text.