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rM. smegmatis-elicited HIV-1-specific CD8+ T cells secreted IFN-γ. Day 7 splenocytes from mice immunized with 107 CFU recombinant mycobacteria expressing gp120 (integrative) were exposed to no peptide, P18, or a gp120 peptide pool and evaluated in an ELISPOT assay. Splenocytes from mice immunized with rM. smegmatis expressing Msp1 were used as a control. The mean (± SEM) spot-forming cells (SFC) per 106 splenocytes for each group of mice (n = 4 per group) is shown.
