TABLE 3.
Relative EGFP mRNA levels in PRV-infected rat brain tissue samples
| Strain | Relative EGFP mRNA level ata:
|
|||||
|---|---|---|---|---|---|---|
| 48 h pi
|
60 h pi
|
96 h pi
|
||||
| Cer | Hyp | Cer | Hyp | Cer | Hyp | |
| PRV151 | 0.01 | 0.95 | 0.21 | 58.21 | — | — |
| PRV99G | 0.01 | 0.86 | 0.16 | 9.56 | — | — |
| PRV152 | Neg | 0.04 | 0.02 | 0.78 | 41.65 | 368.05 |
a
mRNA preparations isolated at the indicated times (pi, postinfection) from cerebellum (Cer) or hypothalamus (Hyp) of three infected rats per time point and per virus strain were combined and quantified by real-time RT-PCR. Results for PRV151 in cerebellum at 48 h postinfection were set to 0.01. —, no sample available; Neg, below RT-PCR detection limit.