TABLE 4.
Validation of microarray data by LightCycler kinetic RT-PCR
| Gene product (designation) | Samplea | Fold increaseb
|
|
|---|---|---|---|
| Microarray | RT-PCR | ||
| Chemokine, C-X-C motif, ligand 10 (Cxcl10) | PRV152, 96 h pi, Hyp | 194.7 | 636.4 |
| Complement component 3 (C3) | PRV152, 96 h pi, Cer | 2.9 | 2.1 |
| Dual-specificity phosphatase 1 (Dusp1) | PRV99G, 60 h pi, Cer | 3.3 | 1.9 |
| Guanylate nucleotide binding protein 2 (Gbp2) | PRV152, 96 h pi, Cer | 13.5 | 4.1 |
| Interferon regulatory factor 7 (Irf7) | PRV151, 60 h pi, Cer | 2.1 | 1.7 |
| Metallothionein 2 (Mt2) | PRV151, 60 h pi, Hyp | 14.8 | 6.8 |
| Myxovirus resistance 1 (Mx1) | PRV152, 96 h pi, Hyp | 50.7 | 298.2 |
| Neurogranin (Nrgn) | PRV152, 60 h pi, Cer | 9.4 | 13.5 |
| Proteosome subunit, beta type 9 (Psmb9) | PRV152, 96 h pi, Hyp | 10.8 | 33.4 |
| Serum/glucocorticoid-regulated kinase (Sgk) | PRV151, 48 h pi, Hyp | 5.4 | 3.7 |
| Serum/glucocorticoid-regulated kinase (Sgk) | PRV99G, 48 h pi, Cer | 3.1 | 2.1 |
a
pi, postinfection; Hyp, hypothalamus; Cer, cerebellum.
b
Comparison of relative increases in mRNA accumulation of 10 selected gene products between respective PRV- and mock-infected samples (set to 1.0) as determined by microarray and RT-PCR analysis.