FIG. 3.

HCV entry is sensitive to concanamycin A (Con A) and NH₄Cl. (A) Huh-7.5 cells were incubated with concanamycin A (0, 12.5, 25, or 50 nM) prior to infection (squares) or at 3 h p.i. (triangles) with HCV. Samples were harvested for luciferase assays at 24 h p.i. The dashed line indicates the background level of the assay from naive Huh-7.5 cells. (B) Huh-7.5 cells were incubated with concanamycin A (0, 12.5, 25, or 50 nM) prior to infection (squares) or at 3 h p.i. (triangles) with HSV-1. Samples were harvested for luciferase assays at 12 h p.i. (C) Huh-7.5 cells were untreated (0 nM) or incubated with 25 mM NH₄Cl prior to infection with HCV (black bars) or HSV-1 (white bars). Samples were harvested for luciferase assays at 24 h p.i. In panels A, B, and C, each point or bar is the average value of triplicate wells; error bars show standard deviations.