FIG. 2.

NS polyprotein expression and processing by infection of HeLa cells with MRKAd5-NSmut and MRKAd6-NSmut. Western blotting of whole-cell extracts from HeLa cells infected at different MOIs with MRKAd5-NSmut or MRKAd6-NSmut. Whole-cell extracts were normalized for total protein content before loading on gel. Mature NS3, NS5A, and NS5B products (indicated by arrows) were detected with specific antibodies. Extract from mock-infected cells was used as a negative control (mock). Molecular weight markers are reported on the left side of each panel.