FIG. 4.

Effect of adenovirus vector boosting injection in rhesus macaques. IFN-γ ELISPOT responses are shown for individual monkeys after priming (T = 8 weeks, gray bars), before boosting (T = 24 weeks, white bars), and after boosting (T = 28 weeks, black bars). Each bar represents the total anti-NS response, calculated by adding up positive responses to individual NS peptide pools and correcting for DMSO background. Results are expressed as the number of IFN-γ SFC per 10⁶ PBMC. Groups A, C, and E were immunized with MRKAd5-NSmut at the indicated doses (top left). Groups B, D, and F were immunized with MRKAd6-NSmut at the indicated doses (top left).