FIG. 7.

Effect of i-leader null and C-terminal-truncation mutants. (A) Genome structures of recombinants containing the i-leader mutations (pm383 or lp53). The i-leader mutations (at nt 7978 and 8299) are indicated. The regions of overlap recombination in recombinant viruses are indicated by dashed lines. (B) Expression of i-leader, L1 52/55K, and E3-ADP proteins. Western blot analysis was carried out with whole-cell lysates prepared at 24 h postinfection. (C). Plaque morphologies of recombinant and parent viruses. Cells were stained 10 days postinfection and photographed.