FIG. 3.

Processing of ASFV polyproteins occurs after their incorporation into the membrane-particulate fraction. Infected Vero cells were pulse-labeled with [³⁵S]methionine-[³⁵S]cysteine for 15 min (P15′ lanes) at 12 hpi and either immediately processed or subsequently chased for 15 min, 1 h, 3 h, 6 h, and 12 h (C15′, C1 h, C3 h, C6 h, and C12 h lanes, respectively). The postnuclear supernatants were fractionated into membrane-particulate (lanes P) and soluble (lanes S) fractions and immunoprecipitated with anti-pp220/p150, anti-pp62/p15, and anti-p72 antibodies. The proteins detected in the pulse and chases after SDS-polyacrylamide gel electrophoresis are indicated.