FIG. 4.

Polyprotein pp62 processing depends on polyprotein pp220 expression. Vero cells were mock infected (lane M) or infected with parental BA71V (lane WT) or recombinant v220i virus in the presence (lane +) or absence (lane −) of IPTG (isopropyl-β-d-thiogalactopyranoside). At 20 hpi, the cells were lysed and analyzed, together with purified ASFV particles (lane V), by Western immunoblotting with anti-pp220/p150 and anti-pp62/p35 antibodies. The positions of polyprotein pp220 and its derived protein p150 (upper panel) and of polyprotein pp62, the intermediate processing precursor pp46, and the mature product p35 (lower panel), are indicated.