FIG. 6.

Proteolytic processing of core polyproteins pp220 and pp62 requires the expression of the major capsid protein p72. Vero cells were mock infected (lane M) or infected for 20 h with parental BA71V (lane WT) or recombinant vA72 virus in the presence (lane +) or absence (lane −) of IPTG. Cell extracts were analyzed, together with purified ASFV particles (lane V), by immunoblotting with antibodies against capsid protein p72, polyprotein pp220 and mature product p150, or polyprotein pp62 and mature product p35. The positions of the detected proteins are indicated.