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Coexpression of polyproteins pp220 and pp62 in COS-7 cells. COS-7 cells were transfected with plasmids bearing the pp220- or pp62-encoding genes or cotransfected with both plasmids and infected with vaccinia virus vTF7-3 in the presence of Ara C. At 7 hpi, the cells were fractionated into a low-speed sediment (lanes P) and a postnuclear supernatant (lanes S) and the fractions were analyzed by Western immunoblotting with anti-pp220 or anti-pp62 serum.
