TABLE 2.
FACS analysis of cell populations in wild-type control and Sn-deficient mice 18 h after intraperitoneal injection of thioglycolate broth
| Substance locationa | Cell populations | % of total cellsb
|
|
|---|---|---|---|
| Sn+/+ | Sn−/− | ||
| Bone marrow (n = 13-15) | T cells (CD3) | 2.6 ± 0.8 | 3.1 ± 1.0 |
| B cells (CD19) | 17.6 ± 2.5 | 22.0 ± 4.2**c | |
| Neutrophils (GR-I+ Siglec E+) | 25.5 ± 5.1 | 25.4 ± 5.9 | |
| Eosinophils (CCR3+ Siglec F+) | 1.0 ± 0.7 | 0.8 ± 0.3 | |
| Blood (n = 13-15) | T cells (CD3) | 23.1 ± 4.3 | 22.0 ± 3.4 |
| B cells (CD19) | 48.9 ± 3.8 | 44.6 ± 9.6 | |
| NK cells (NK 1.1) | 8.3 ± 2.0 | 9.3 ± 2.9 | |
| Neutrophils (GR-I+ Siglec E+) | 7.3 ± 3.2 | 8.0 ± 3.5 | |
| Eosinophils (CCR3+ Siglec F+) | 0.3 ± 0.2 | 0.4 ± 0.2 | |
| Peritoneum (n = 13-15) | T cells (CD3) | 3.3 ± 1.0 | 3.8 ± 2.1 |
| B cells (CD19) | 15.1 ± 4.3 | 14.0 ± 5.8 | |
| NK cells (NK 1.1) | 16.5 ± 5.1 | 16.3 ± 3.2 | |
| Neutrophils (GR-I+ Siglec E+) | 22.7 ± 7.7 | 29.8 ± 8.7* | |
| Monocytes (GR-I+ F4/80+) | 17.5 ± 3.4 | 18.6 ± 6.8 | |
| Eosinophils (CCR3+ Siglec F+) | 24.9 ± 4.1 | 18.8 ± 5.3** | |
a
Total numbers of leukocytes recovered were 2.1 × 107 ± 5.3 × 106 and 1.8 × 107 ± 8.2 × 106 from bone marrow and 1.6 × 107 ± 3.8 × 106 and 1.6 × 107 ± 5.1 × 106 from peritoneal cavity for Sn+/+ and Sn−/− mice, respectively.
b
Data are given as the mean percentage of total cells ± SD from three independent experiments.
c
Asterisks indicate the degree of significance applying the t test: *, P <0.05; **, P <0.01; ***, P <0.001.