TABLE 2.
Ectopic proliferation and cell type distribution in P14 Rb1654/− retinaea
| Cell typeb | Ectopic proliferation, cell type distributionc in the retinae of individual mice of the indicated genotype
|
|||
|---|---|---|---|---|
| Chx10-cre; Rb1654/+ | Chx10-cre; Rb1+/− | Chx10-cre; Rb1Lox/654 | Chx10-cre; Rb1Lox/654 | |
| BrdU+ | >0/250, 0/250 (0) | 0/250, 0/250 (0) | 5/250, 4/250 (1.8 ± 0.3) | 11/250, 6/250 (3.4 ± 1.4) |
| Rhod+ | 145/250, 130/250 (55 ± 4.2) | 151/250, 122/250 (54 ± 8.2) | 65/250, 55/250 (24 ± 2.8) | 78/250, 69/250 (29 ± 2.5) |
| Recov+ | 139/250, 127/250 (53 ± 3.3) | 168/250, 150/250 (64 ± 5.1) | 64/250, 68/250 (26 ± 1.1) | 23/250, 27/250 (10 ± 1.1) |
| Cone+ | 6/250, 4/250 (2 ± 0.5) | 7/250, 4/250 (2.2 ± 0.8) | 7/250, 5/250 (2.4 ± 0.5) | 6/250, 7/250 (2.6 ± 0.2) |
| PKCα+ | 10/250, 9/250 (3.8 ± 0.3) | 8/250, 6/250 (2.8 ± 0.5) | 8/250, 11/250 (3.8 ± 0.8) | 10/250, 9/250 (3.8 ± 0.2) |
| Chx10+ | 19/250, 17/250 (7.2 ± 0.5) | 12/250, 16/250 (5.6 ± 1.1) | 27/250, 22/250 (9.8 ± 1.4) | 32/250, 28/250 (12 ± 1.1) |
| Pax6+ | 11/250, 9/250 (4 ± 0.5) | 6/250, 8/250 (2.8 ± 0.5) | 24/250, 23/250 (9.4 ± 0.2); 16% ± 2%d | 23/250, 20/250 (8.6 ± 0.8); 12% ± 3%d |
| Syntax+ | 20/250, 18/250 (7.6 ± 0.5) | 14/250, 11/250 (5 ± 0.8) | 11/250, 9/250 (4 ± 0.5) | 5/250, 7/250 (2.4 ± 0.5) |
| Calb+ | 1/500, 2/500 (0.3 ± 0.1) | 1/500, 0/500 (0.1 ± 0.1) | 1/500, 0/500 (0.1 ± 0.1) | 1/500, 0/500 (0.1 ± 0.1) |
| GluSyn+ | 4/250, 5/250 (1.8 ± 0.3) | 5/250, 6/250 (2.2 ± 0.3) | 17/250, 13/250 (6.0 ± 1) | 22/250, 27/250 (9.8 ± 0.1.4) |
Mice received an intraperitoneal injection of [3H]thymidine (5 μCi/g) 1 hour prior to retinal dissection. [3H]thymidine+ cells expressed Chx10 (∼1 to 2% [3H]thymidine+, immunopositive/[3H]thymidine+) and Pax6.
Rhod, rhodopsin; recov, recovesin; PKCα, protein kinase C α; calb, calbindin; GluSyn, glutamine synthetase; syntax, syntaxin; cone, cone arrestin.
Indicated cell type/total (% mean ± % standard deviation).
The fraction of proliferating cells expressing Pax6 ([3H]thymidine+, immunopositive/[3H]thymidine+) is indicated. The number of grains for 10 randomly selected [3H]thymidine-labeled cells varied from 10 to 27 grains per cell (mean, 14 ± 10). The number of grains for 10 randomly selected unlabeled cells varied from 0 to 3.