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. 2006 Feb;26(4):1259–1271. doi: 10.1128/MCB.26.4.1259-1271.2006

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Copyright © 2006, American Society for Microbiology

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FIG. 4. — Histone H3 phosphorylation and acetylation by Npm. (A) Immunoblotting demonstrating histone H3 phosphorylation and acetylation in F9 nuclei by 5 μM rNpm. Total histone H3 was used as a loading control. The figures were prepared from more than one membrane in panels A and E because of the significantly diminished signal due to the repeated stripping of the membrane. (B) Immnofluorescence shows that H3S10 and H3S28 were globally phosphorylated when F9 nuclei were incubated with 5 μM rNpm. Bar, 3 μm. (C) Time course of histone H3 phosphorylation in F9 nuclei incubated with 5 μM rNpm. (D) Okadaic acid (OA) unmasked p-H3S10 in F9 nuclei incubated in egg extract. (E) Immunoblotting shows inhibition of histone H3 phosphorylation and chromatin decondensation by staurosporine. ac-H3K14 was not inhibited by staurosporine. (F) PKA phosphorylated histone H3 when incubated with F9 nuclei but could not induce centromeric heterochromatin decondensation without rNpm.