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. 2006 Feb;26(4):1259–1271. doi: 10.1128/MCB.26.4.1259-1271.2006

FIG. 5.

FIG. 5.

Npm releases heterochromatin proteins from F9 nuclei. (A) HP1β and TIFβ were lost from F9 nuclei by Npm as shown by immunoblotting. (B) Immunofluorescence confirmed loss of HP1β and TIF1β from F9 nuclei by rNpm. Bar, 5 μm. (C) Dose-dependent histone H3 modifications by various forms of rNpm. Each rNpm was used at 5, 1.5, and 0.5 μM (black triangles). (D) Summary of epigenetic modification in F9 nuclei incubated with various forms of rNpm. The scores (− to ++) are intended to show overall tendency, not exactly quantitative values.