TABLE 3.
Increase in Ag43 protein levels in rfaH mutants compared to the corresponding wild-type strains
Strain background | Relative expression of Ag43 variantsa
|
||
---|---|---|---|
Flu | ORF52III | ORF47V | |
MG1655 (wild type) | 1 | ||
MG1655rfaH | 2.75 | ||
536 (wild type) | 1 | 1 | |
536ORF52III | 6.48 | ||
536ORF47V | 6.50 | ||
536rfaH | 2.26 | 17.02 | |
536rfaH ORF52III | 11.94 | ||
536rfaH ORF47V | 3.73 |
The signal intensity of an Ag43 variant was normalized to the signal intensity of the same Ag43 variant obtained for the wild-type strain.