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. 2006 Feb;188(4):1567–1576. doi: 10.1128/JB.188.4.1567-1576.2006

FIG. 7.

FIG. 7.

Electrophoretic mobility shift. Electrophoretic mobility shift assay of HbrL-H6 using hemA, hemB, hemZ, hbrL, hemCE, and hemH probes is shown. (A) Electrophoretic mobility of hemB probe with increasing amounts of HbrL lysate added in the presence and absence of 7 μM hemin is shown. Ten micrograms of Bl21(DE3) lysate was added to lanes marked “0.” (B) Electrophoretic mobility of hemA probe with increasing amounts of HbrL lysate added in the presence and absence of 7 μM hemin is shown. (C) Electrophoretic mobility of hemZ probe with increasing amounts of HbrL lysate added in the presence and absence of 7 μM hemin is shown. (D) Electrophoretic mobility of hbrL probe in the presence of 7 μM hemin with increasing amounts of HbrL lysate added is shown. (E) Electrophoretic mobility of hemCE probe in the presence of 7 μM hemin with increasing amounts of HbrL lysate added is shown. (F) Electrophoretic mobility of hemH probe in the presence of 7 μM hemin with increasing amounts of HbrL lysate added is shown.