Restriction and physical map of the 13-kb spnTIR locus in S. marcescens SS-1. NI, NsiI; EI, EcoRI; EV, EcoRV; BI, BamHI; KI, KpnI; SI, SmaI; PI, PstI. The dashed arrow indicates the disrupted transposase gene. The gray box represents the DNA fragment identified in a previous study (15). The double-headed arrow indicates the region of TnTIR. Scale bar, 1 kb.